Natural Killer (NK) and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

Chimeric antigen receptor (CAR)-modified immune cell therapy has become an emerging treatment for cancers and infectious diseases. NK-based immunotherapy, particularly CAR-NK cell, is one of the most promising 'off-the-shelf' development without severe life-threatening toxicity. However, bottleneck developing a successful achieving sufficient numbers non-exhaustive, long-lived, cells from third party. Here, we developed new expansion method using Epstein-Barr virus- (EBV) transformed B line expressing genetically modified membrane form interleukin-21 (IL-21). In this protocol, step-by-step procedures are provided to expand NK cord blood peripheral blood, as well solid organ tissues. This work will significantly enhance clinical immunotherapy.